Multiplex PCR and Microarray for Detection of Swine Respiratory Pathogens.
Identifieur interne : 000774 ( Main/Exploration ); précédent : 000773; suivant : 000775Multiplex PCR and Microarray for Detection of Swine Respiratory Pathogens.
Auteurs : O. Lung [Canada] ; S. Ohene-Adjei [Canada] ; C. Buchanan [Canada] ; T. Joseph [Canada] ; R. King [Canada] ; A. Erickson [Canada] ; S. Detmer [Canada] ; A. Ambagala [Canada]Source :
- Transboundary and emerging diseases [ 1865-1682 ] ; 2017.
Descripteurs français
- KwdFr :
- Analyse par réseau de protéines (), Analyse par réseau de protéines (médecine vétérinaire), Animaux, Bactéries (), Bactéries (isolement et purification), Maladies des porcs (diagnostic), Maladies des porcs (microbiologie), Maladies des porcs (virologie), Réaction de polymérisation en chaine multiplex (médecine vétérinaire), Sensibilité et spécificité, Suidae, Virus (), Virus (isolement et purification).
- MESH :
- diagnostic : Maladies des porcs.
- isolement et purification : Bactéries, Virus.
- microbiologie : Maladies des porcs.
- médecine vétérinaire : Analyse par réseau de protéines, Réaction de polymérisation en chaine multiplex.
- virologie : Maladies des porcs.
- Analyse par réseau de protéines, Animaux, Bactéries, Sensibilité et spécificité, Suidae, Virus.
English descriptors
- KwdEn :
- Animals, Bacteria (classification), Bacteria (isolation & purification), Multiplex Polymerase Chain Reaction (veterinary), Protein Array Analysis (methods), Protein Array Analysis (veterinary), Sensitivity and Specificity, Swine, Swine Diseases (diagnosis), Swine Diseases (microbiology), Swine Diseases (virology), Viruses (classification), Viruses (isolation & purification).
- MESH :
- classification : Bacteria, Viruses.
- diagnosis : Swine Diseases.
- isolation & purification : Bacteria, Viruses.
- methods : Protein Array Analysis.
- microbiology : Swine Diseases.
- veterinary : Multiplex Polymerase Chain Reaction, Protein Array Analysis.
- virology : Swine Diseases.
- Animals, Sensitivity and Specificity, Swine.
Abstract
Porcine respiratory disease complex (PRDC) is one of the most important health concerns for pig producers and can involve multiple viral and bacterial pathogens. No simple, single-reaction diagnostic test currently exists for the simultaneous detection of major pathogens commonly associated with PRDC. Furthermore, the detection of most of the bacterial pathogens implicated in PRDC currently requires time-consuming culture-based methods that can take several days to obtain results. In this study, a novel prototype automated microarray that integrates and automates all steps of post-PCR microarray processing for the simultaneous detection and typing of eight bacteria and viruses commonly associated with PRDC is described along with associated multiplex reverse transcriptase PCR. The user-friendly assay detected and differentiated between four viruses [porcine reproductive and respiratory syndrome virus (PRRSV), influenza A virus, porcine circovirus type 2, porcine respiratory corona virus], four bacteria (Mycoplasma hyopneumoniae, Pasteurella multocida, Salmonella enterica serovar Choleraesuis, Streptococcus suis), and further differentiated between type 1 and type 2 PRRSV as well as toxigenic and non-toxigenic P. multocida. The assay accurately identified and typed a panel of 34 strains representing the eight targeted pathogens and was negative when tested with 34 relevant and/or closely related non-target bacterial and viral species. All targets were also identified singly or in combination in a panel of clinical lung samples and/or experimentally inoculated biological material.
DOI: 10.1111/tbed.12449
PubMed: 26662640
Affiliations:
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Le document en format XML
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Lung</name><affiliation wicri:level="1"><nlm:affiliation>Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food Inspection Agency, Lethbridge, AB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food Inspection Agency, Lethbridge, AB</wicri:regionArea><wicri:noRegion>AB</wicri:noRegion></affiliation></author><author><name sortKey="Ohene Adjei, S" sort="Ohene Adjei, S" uniqKey="Ohene Adjei S" first="S" last="Ohene-Adjei">S. Ohene-Adjei</name><affiliation wicri:level="1"><nlm:affiliation>Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food Inspection Agency, Lethbridge, AB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food Inspection Agency, Lethbridge, AB</wicri:regionArea><wicri:noRegion>AB</wicri:noRegion></affiliation></author><author><name sortKey="Buchanan, C" sort="Buchanan, C" uniqKey="Buchanan C" first="C" last="Buchanan">C. Buchanan</name><affiliation wicri:level="1"><nlm:affiliation>Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food Inspection Agency, Lethbridge, AB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food Inspection Agency, Lethbridge, AB</wicri:regionArea><wicri:noRegion>AB</wicri:noRegion></affiliation></author><author><name sortKey="Joseph, T" sort="Joseph, T" uniqKey="Joseph T" first="T" last="Joseph">T. Joseph</name><affiliation wicri:level="1"><nlm:affiliation>Animal Health Centre, BC Ministry of Agriculture, Abbotsford, BC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Animal Health Centre, BC Ministry of Agriculture, Abbotsford, BC</wicri:regionArea><wicri:noRegion>BC</wicri:noRegion></affiliation></author><author><name sortKey="King, R" sort="King, R" uniqKey="King R" first="R" last="King">R. King</name><affiliation wicri:level="1"><nlm:affiliation>Animal Health and Assurance Division, Alberta Agriculture and Rural Development, Edmonton, AB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Animal Health and Assurance Division, Alberta Agriculture and Rural Development, Edmonton, AB</wicri:regionArea><wicri:noRegion>AB</wicri:noRegion></affiliation></author><author><name sortKey="Erickson, A" sort="Erickson, A" uniqKey="Erickson A" first="A" last="Erickson">A. Erickson</name><affiliation wicri:level="1"><nlm:affiliation>Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food Inspection Agency, Lethbridge, AB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food Inspection Agency, Lethbridge, AB</wicri:regionArea><wicri:noRegion>AB</wicri:noRegion></affiliation></author><author><name sortKey="Detmer, S" sort="Detmer, S" uniqKey="Detmer S" first="S" last="Detmer">S. Detmer</name><affiliation wicri:level="1"><nlm:affiliation>Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK</wicri:regionArea><wicri:noRegion>SK</wicri:noRegion></affiliation></author><author><name sortKey="Ambagala, A" sort="Ambagala, A" uniqKey="Ambagala A" first="A" last="Ambagala">A. Ambagala</name><affiliation wicri:level="1"><nlm:affiliation>Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food Inspection Agency, Lethbridge, AB, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food Inspection Agency, Lethbridge, AB</wicri:regionArea><wicri:noRegion>AB</wicri:noRegion></affiliation></author></analytic><series><title level="j">Transboundary and emerging diseases</title><idno type="eISSN">1865-1682</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Bacteria (classification)</term><term>Bacteria (isolation & purification)</term><term>Multiplex Polymerase Chain Reaction (veterinary)</term><term>Protein Array Analysis (methods)</term><term>Protein Array Analysis (veterinary)</term><term>Sensitivity and Specificity</term><term>Swine</term><term>Swine Diseases (diagnosis)</term><term>Swine Diseases (microbiology)</term><term>Swine Diseases (virology)</term><term>Viruses (classification)</term><term>Viruses (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse par réseau de protéines ()</term><term>Analyse par réseau de protéines (médecine vétérinaire)</term><term>Animaux</term><term>Bactéries ()</term><term>Bactéries (isolement et purification)</term><term>Maladies des porcs (diagnostic)</term><term>Maladies des porcs (microbiologie)</term><term>Maladies des porcs (virologie)</term><term>Réaction de polymérisation en chaine multiplex (médecine vétérinaire)</term><term>Sensibilité et spécificité</term><term>Suidae</term><term>Virus ()</term><term>Virus (isolement et purification)</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Bacteria</term><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Swine Diseases</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Maladies des porcs</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacteria</term><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Bactéries</term><term>Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Protein Array Analysis</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des porcs</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Swine Diseases</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Analyse par réseau de protéines</term><term>Réaction de polymérisation en chaine multiplex</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Multiplex Polymerase Chain Reaction</term><term>Protein Array Analysis</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Maladies des porcs</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Swine Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Sensitivity and Specificity</term><term>Swine</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse par réseau de protéines</term><term>Animaux</term><term>Bactéries</term><term>Sensibilité et spécificité</term><term>Suidae</term><term>Virus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Porcine respiratory disease complex (PRDC) is one of the most important health concerns for pig producers and can involve multiple viral and bacterial pathogens. No simple, single-reaction diagnostic test currently exists for the simultaneous detection of major pathogens commonly associated with PRDC. Furthermore, the detection of most of the bacterial pathogens implicated in PRDC currently requires time-consuming culture-based methods that can take several days to obtain results. In this study, a novel prototype automated microarray that integrates and automates all steps of post-PCR microarray processing for the simultaneous detection and typing of eight bacteria and viruses commonly associated with PRDC is described along with associated multiplex reverse transcriptase PCR. The user-friendly assay detected and differentiated between four viruses [porcine reproductive and respiratory syndrome virus (PRRSV), influenza A virus, porcine circovirus type 2, porcine respiratory corona virus], four bacteria (Mycoplasma hyopneumoniae, Pasteurella multocida, Salmonella enterica serovar Choleraesuis, Streptococcus suis), and further differentiated between type 1 and type 2 PRRSV as well as toxigenic and non-toxigenic P. multocida. The assay accurately identified and typed a panel of 34 strains representing the eight targeted pathogens and was negative when tested with 34 relevant and/or closely related non-target bacterial and viral species. All targets were also identified singly or in combination in a panel of clinical lung samples and/or experimentally inoculated biological material.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Lung, O" sort="Lung, O" uniqKey="Lung O" first="O" last="Lung">O. Lung</name></noRegion><name sortKey="Ambagala, A" sort="Ambagala, A" uniqKey="Ambagala A" first="A" last="Ambagala">A. Ambagala</name><name sortKey="Buchanan, C" sort="Buchanan, C" uniqKey="Buchanan C" first="C" last="Buchanan">C. Buchanan</name><name sortKey="Detmer, S" sort="Detmer, S" uniqKey="Detmer S" first="S" last="Detmer">S. Detmer</name><name sortKey="Erickson, A" sort="Erickson, A" uniqKey="Erickson A" first="A" last="Erickson">A. Erickson</name><name sortKey="Joseph, T" sort="Joseph, T" uniqKey="Joseph T" first="T" last="Joseph">T. Joseph</name><name sortKey="King, R" sort="King, R" uniqKey="King R" first="R" last="King">R. King</name><name sortKey="Ohene Adjei, S" sort="Ohene Adjei, S" uniqKey="Ohene Adjei S" first="S" last="Ohene-Adjei">S. Ohene-Adjei</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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